recombinant human saa1  (Novoprotein)

Journal: Cell metabolism

Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

doi: 10.1016/j.cmet.2025.12.008

Figure Lengend Snippet: (A) Cell-cell communication analysis of SAA signaling pathway for the indicated depots and cells. Line colors and widths represent the sending cell and the strength of the signal, respectively. (B) Pseudobulk comparison of differentially expressed genes in epiploic vs. subcutaneous depots, highlighting the indicated signaling pathways. (C) Expression of SAA1 mRNA upon stimulation with LPS (10 ng/mL), TNF-α (2.5 ng/mL), IL-1β (10 ng/mL), or IL-6 (10 ng/mL) for 24 h. Data are presented as fold change (FC) over vehicle. Significant (<0.05) p values compared with the vehicle from one-way ANOVA are shown. (D) Relative expression of SAA1 (left) and SAA2 (right) mRNA in adipocytes, ASPCs, adipose-derived endothelial cells (adECs), and THP1-derived macrophages (THP1 M0). Significant (<0.05) p values compared with the cell-type-specific control from one-way ANOVA are shown. (E) Results from ELISA measurements of SAA in conditioned media from human adipocytes incubated without or with LPS for 72 h. Results are displayed as mean, error bars represent standard error of the mean, and statistical significance was calculated using Student’s t test. (F) Analyses of DNA methylation in the indicated cell classes for the promoter and gene body regions of SAA1 and SAA2 , respectively. Chromosomal localization and motifs for NFKB2/RELA/REL and STAT3 are indicated for both genes. (G) SAA1 and SAA2 mRNA expression in adipocytes treated with or without LPS in the presence or absence of different inhibitors described in the main text. Data are presented as FC over vehicle. Significant (<0.05) p values compared with the vehicle from one-way ANOVA are shown. For (C), (D), and (G), data are displayed as geometric mean ± 95% confidence intervals with technical replicates displayed from three independent experiments. ASPC, adipose stromal and progenitor cell.

Article Snippet: Human recombinant SAA1 protein , OriGene , Cat#TP310664.

Techniques: Comparison, Protein-Protein interactions, Expressing, Derivative Assay, Control, Enzyme-linked Immunosorbent Assay, Incubation, DNA Methylation Assay

Journal: Cell metabolism

Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

doi: 10.1016/j.cmet.2025.12.008

Figure Lengend Snippet: (A) Top Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways based on differentially expressed genes from comparisons of SAA1- or LPS-treated vs. control-treated adipose-derived stromal-vascular cells. (B) Network view of the six common SAA1- and LPS-regulated pathways showing shared and treatment-specific genes. (C) Expression scores for SAA1- (left) and LPS-responsive (right) genes following a 6-h incubation with either ligand in stromal-vascular cells from subcutaneous human WAT. Results are displayed on the uniform manifold approximation and projection panel from and violin plots for each indicated cell class. Note that adipocytes and mesothelial cells were removed as these are not present in the stromal-vascular fraction from the subcutaneous depot. (D) Representative immunostaining of epiploic adipose tissue using antibodies directed against markers for Adipo SAA (CES1) and macrophages (CD68). Hoechst33342 was used as the counterstain for nuclei. Scale bar, 100 μm. ASPC, adipose stromal and progenitor cell; NES, normalized enrichment score.

Article Snippet: Human recombinant SAA1 protein , OriGene , Cat#TP310664.

Techniques: Control, Derivative Assay, Expressing, Incubation, Immunostaining

Journal: The Journal of Clinical Investigation

Article Title: SAA1/FPR2 signaling between keratinocytes and neutrophils sustains chronic inflammation in Sweet syndrome

doi: 10.1172/JCI193566

Figure Lengend Snippet: ( A ) Ligand-receptor analysis reveals keratinocyte- and neutrophil-specific interactions. Keratinocytes expressed SAA1 transcripts and neutrophils expressed the FPR2 receptor (red dot, right-most column). ( B ) Dot plot demonstrating predominantly cell-specific expression of SAA1 and FPR2 transcripts. The dot size reflects the percentage of cells expressing the gene, and the color illustrates the level of gene expression. ( C ) Representative immunofluorescence staining images and quantification from 5 diseased and 5 control samples, confirming the expression of SAA1 and FPR2 in keratinocytes and neutrophils, respectively. Scale bars: 100 μm. ( D ) Dot plot comparing keratinocyte SAA1 and the control gene DEFB1 in different inflammatory skin conditions. The dot size reflects the percentage of cells expressing the gene, and the color illustrates the level of gene expression. ( E ) SAA1 secretion measured by ELISA in healthy neutrophils or healthy neutrophils exposed to keratinocytes. ( F ) Antibodies blocking SAA1 and FPR2 restored long-lived neutrophil lifespan to WT neutrophil levels. ( G ) Recombinant human SAA1 increased neutrophil survival at 72 hours ( n = 3 independent donors). Data indicate the mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed, unpaired Student’s t test ( E and G ) and 1-way ANOVA with individual comparisons ( F ).

Article Snippet: Freshly isolated neutrophils from healthy donors were treated with recombinant human SAA1 (SAA1-257H, Creative Biomart).

Techniques: Expressing, Gene Expression, Immunofluorescence, Staining, Control, Enzyme-linked Immunosorbent Assay, Blocking Assay, Recombinant

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: siRNA sequences

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques:

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: RT qPCR primer sequences

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Quantitative RT-PCR

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: SAA1 is highly expressed in ESCC tissues and cell lines. A SAA1 mRNA expression in ESCC tissues in TCGA and GEO datasets was analyzed. B SAA1 protein expression in ESCC tissues determined by western blot analysis (n = 16). C SAA1 mRNA and protein expression in HEEC cells and ESCC cell lines was determined by RT‒qPCR. D The cDNA sequencing results of SAA1 in two ESCC cell lines showed that a single nucleotide polymorphism was observed at nucleotide position 209, as indicated by the arrows. **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Expressing, Western Blot, Sequencing

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: SAA1 promotes the proliferation and migration of TE-1 cells in vitro and in vivo. A TE-1 cells were stimulated with different concentrations of rhSAA1 for 72 h, and cell proliferation was determined by CCK-8 assay. B TE-1 cells were stimulated with 10 μg/mL rhSAA1 for 24 h, and the migration of cells was determined by wound healing assay. C SAA1 was overexpressed in TE-1 cells, and the overexpression efficiencies were confirmed by RT‒qPCR and western blotting. D The proliferation of SAA1-overexpressing TE-1 cells was determined by CCK-8 assay. E The migration of SAA1-overexpressing TE-1 cells was determined by wound healing assay. F The colony formation capacity of SAA1-overexpressing TE-1 cells was determined by colony formation assay. G Xenograft tumors were photographed. H The volume of xenograft tumors was determined at the end of the experiment. I Xenografts were weighed at the end of the experiment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Migration, In Vitro, In Vivo, CCK-8 Assay, Wound Healing Assay, Over Expression, Western Blot, Colony Assay

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: SAA1 knockdown inhibits proliferation and migration, and promotes the apoptosis of ESCC cells. A The expression of SAA1 was knocked down in Eca109 cells, and the knockdown efficiencies were confirmed by RT‒qPCR and western blotting. B The proliferation of SAA1-knockdown Eca109 cells was determined by CCK-8 assay. C The migration of SAA1-knockdown Eca109 cells was determined by wound healing assay. D The apoptosis of SAA1-knockdown Eca109 cells was determined by flow cytometry. *p < 0.05, **p < 0.01, ****p < 0.0001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Knockdown, Migration, Expressing, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: SAA1 induces β-catenin phosphorylation at Ser675. A The protein levels of total β-catenin, pSer675-β-catenin, MMP-9 and c-Myc in SAA1-overexpressing TE-1 cells were detected by western blotting. B The protein levels of total β-catenin, pSer675-β-catenin, MMP-9 and c-Myc in SAA1-knockdown Eca109 cells were detected by western blotting. C TE-1 cells and Eca109 cells were stained with anti-pSer675-β-catenin, anti-β-catenin and DAPI and observed by confocal fluorescence microscopy, scale bar = 20 μm. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Phospho-proteomics, Western Blot, Knockdown, Staining, Fluorescence, Microscopy

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: The β-catenin inhibitor XAV939 alleviates SAA1 overexpression-mediated proliferation and migration. A SAA1-overexpressing TE-1 cells were treated with XAV939 (10 µmol/L) for 24 h. The protein levels of total β-catenin, pSer675-β-catenin and c-Myc were detected by western blotting. B The proliferation of SAA1-overexpressing TE-1 cells treated with XAV939 (10 µmol/L) was determined by CCK-8 assay. C The migration of SAA1-overexpressing TE-1 cells treated with XAV939 (10 µmol/L) was determined by wound healing assay. **p < 0.01, ***p < 0.001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Over Expression, Migration, Western Blot, CCK-8 Assay, Wound Healing Assay

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: S1P/S1PR1 increases SAA1 expression levels and β-catenin phosphorylation at Ser675 in ESCC cells. A ESCC cells were stimulated with different concentrations of S1P (0, 10, 100, and 1000 nmol/L) for 24 h. SAA1, total β-catenin and pSer675-β-catenin protein levels were detected by western blotting. B ESCC cells were treated with VPC23019 (1000 nmol/L) and then stimulated with S1P (1000 nmol/L) for 24 h. The levels of SAA1, total β-catenin and pSer675-β-catenin were detected by western blotting. C The expression of S1PR1 and S1PR3 proteins in HEEC cells and two ESCC cell lines was detected by western blotting. D The efficiency of S1PR1 overexpression was determined by RT‒qPCR and western blotting. E The efficiency of S1PR1 knockdown was determined by RT‒qPCR and western blot. F The protein levels of SAA1, total β-catenin and pSer675-β-catenin were detected by western blot after S1PR1 overexpression and knockdown in ESCC cells. **p < 0.01, ****p < 0.0001

Article Snippet: In the recombinant human SAA1 (rhSAA1, PeproTech, USA) stimulation assay, TE-1 cells were stimulated with 0, 0.1, 1, and 10 μg/mL rhSAA1, and cell proliferation was monitored every 24 h.

Techniques: Expressing, Phospho-proteomics, Western Blot, Over Expression, Knockdown

Journal: Arthritis Research & Therapy

Article Title: Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis

doi: 10.1186/s13075-023-03243-z

Figure Lengend Snippet: Plasma concentration of SPP1, SAA1, and KNG1 in MDA5 + DM RP-ILD. A–C The concentrations of SPP1, SAA1, and KNG1 in the plasma from an independent cohort were determined by ELISA. P values were calculated by the ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. D–F ROC curve showed the AUC of the 3 verified proteins, respectively

Article Snippet: Following the manufacturer’s instructions, commercial ELISA kits were utilized for quantifying the levels of serum amyloid A1 (SAA1) (Boster Bio, China), V-Set And Immunoglobulin Domain Containing 4 (VSIG4), Coagulation Factor XI (F11) (both from CUSABIO, China), Coagulation Factor XIII A Chain (F13A1), Secreted phosphoprotein 1 (SPP1) and Kininogen 1 (KNG1) (all from Uscn life Science, China) in plasma samples from both patients and normal control subjects.

Techniques: Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay